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The floristic studies on the flora of Akot tahsil of Akola districts, Maharashtra were conducted to assess the potentiality of the plant resources. The study reveals that about 31 plants belonging to 27 families are used in the treatment of kidney stone in local remedies. The information on the medicinal uses of plants is based on the extensive interviews from local healers and herbalists practicing traditional system of medicine. The details of the plant parts used, doses, mode of administration have been reported. Pedilanthes tithymoides and Bryophyllum pinnatum, Tribullus terrestris, and Boerhaavia diffulsa are most effective and commonly used in the treatment of urinary tract diseases and kidney stones. Such plants may prove precious potential source of biologically active compounds for therapeutic use for the treatment of kidney ailments.