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The antimicrobial activity of several extracts of the seeds of Brassica alba L., Brassica campestris L. and Brassica juncea L. was investigated using disk diffusion method against seven reference microorganisms (five bacterial and two fungal strains). The methanolic extracts of all the three species were showed the maximum inhibitory whereas the ethyl acetate, chloroform and petroleum ether extracts of B. alba, B. campestris and B. juncea respectively exhibited a good antibacterial activity against four bacterial strains i. e. S. aureus, B. cereus, P. aeruginosa and S. epidermidis with the diameters of growth inhibition area in the range of 05 – 25 mm. Neither the n–hexane and benzene extracts nor the aqueous extracts of all the three species showed any antimicrobial activity against the tested microorganisms. No antifungal activity was seen with any of the extracts. The results of this study support the use of these species in Indian traditional medicine to treat skin infections.