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Since the advent of antibiotics, pathogenic microorganisms have constantly developed mechanisms to escape the lethal action of these inhibitors. Some of these microorganisms have the methylerythritolphosphate (MEP) pathway, a metabolic pathway for isoprenoid biosynthesis, essential for their survival but absent in humans. In this study, we evaluated the behavior of two commercial strains of Escherichia coli (JM109 and MG1655) during their growth phase in the presence of two inhibitors, fosfomycin (FFM) and fosmidomycin (FSM). Escherichia coli strains were grown in Luria-Bertani (LB) broth supplemented with kanamycin (25 μg/ml) and ampicillin (100 μg/ml). The optical density at 600 nm of each bacterial culture was determined before and after addition of FFM (100 μM) and FSM (100 μM) in presence or not of glucose-6-phosphate (70 μM). Statistical tests were conducted to further explain the effect of inhibitors. The minimum inhibitory concentration of the two antibiotics on the two strains is 20 µM. In LB medium, the response time to growth inhibition of E. coli JM109 and E. coli MG1655 induced by FSM is greater than that of FFM. At advanced stages of bacterial growth, FFM inhibited bacterial growth, which is not the case with FSM. From a general point of view, there is no significant difference between the action of the two antibiotics (p > 0.05).